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	Curriculum Vitae
Dr. Willhite's Homepage	David Grant Willhite May 2005 Personal Data Business Address: Tennessee Wesleyan College P.O. Box 40 Athens, TN 37303 Home Address: 162 County Rd. 578 Englewood, TN 37329 Phone: (423) 746-5236 (W) (423) 507-0086 (H) Email: willhite@twcnet.edu Date of Birth: June 22, 1973 Place: Knoxville, TN Academic Preparation Ph.D. in Biology, University of Cincinnati, 2001 Concentrations: Comparative immunology, cell biology, protein biochemistry Advisor: Dr. Richard D. Karp B. A. in Biology, Carson-Newman College, Jefferson City, TN, 1995 Minor in Sociology Teaching Experience Assistant Professor of Biology, Tennessee Wesleyan College , January 2002-present Cell Biology, Molecular Biology, Genetics, Microbiology Teaching Assistant, University of Cincinnati, 1996-2001 Freshman (Cell biology, genetics, and ecology) and Sophomore (cell biology) laboratories, Head Teaching Assistant, University of Cincinnati, 1997-2000 Freshman laboratory Instructor, Cincinnati School of Medical Massage, 1999-2001 Anatomy and Physiology Courses Taught/Developed Tennessee Wesleyan College Introductory Cell Biology, B 111 General Microbiology, B 265 Principles of Genetics, B 270 Immunology, B 355 Introduction to Bioinformatics, B 421 Cellular and Molecular Biology, B 463 Seminar in Biology, B 471 Cincinnati School of Medical Massage Anatomy and Physiology Research Experience Enhancing Research in Appalachia (ERIA) Summer Research Project 2004 Directed undergraduate research into the localization of the yeast GPCR, Ste2p to rafts. Mentored two students in protein isolation, concentration determination, Western blotting, etc. Enhancing Research in Appalachia (ERIA) Summer Research Project 2003 Directed undergraduate research into the effect of lipid environment on the yeast GPCR, Ste2p. Mentored two students in protein isolation, concentration determination, enzymatic digestion, Western blotting, etc. ERIA Summer Research Project 2002 Directed undergraduate research into the effect of lipid environment on the yeast GPCR, Ste2p. Mentored two students in Western blotting, protein concentration determination, affinity chromatography, etc. Graduate research Determination of protein factor involved in the adaptive immune response to soluble antigen in the American cockroach. Current Research Interests Directing undergraduate research into the effects of lipid-protein interactions on the function of a yeast G-protein coupled receptor (in collaboration with Dr. Stephen Wright, Carson-Newman College and Dr. Terry Bunde, Maryville College). Publications Editor, Freshman Biology manual Miller, Iain. (1999) Biology 113/Biology 183 Supplementary Manual. Hayden-McNeil. In preparation: Wright S, Willhite G, Cannon K, Thomas H, Coughran J, Dalton M, Benton D, Nichting P, Service S, and Bunde T Effects of a combination of mating pheromone and azoles on growth arrest in Saccharomyces cerevisiae. Willhite G, Bunde T, and Wright S. A biochemistry lab series utilizing techniques for isolation and characterization of detergent insoluble membrane fractions (rafts) in Saccharomyces cerevisiae. Willhite DG, Duwel-Eby L, and Karp RD. Demonstration of antigen binding activity by native hemolymph proteins and MALDI identification of reduced proteins associated with the adaptive immune response in the American cockroach, Periplaneta americana Research Techniques Electrophoretic analysis, 2-D electrophoresis, western blotting, affinity chromatography, MALDI mass spectrometry, enzyme analysis, glycoprotein analysis *Presentations: ( = presenter; # = TWC student)** Ergosterol as a raft component and its role in the activity of a yeast GPCR SE Wright, T Bunde, G Willhite. SCB 44th Annual Meeting; December 4-8, 2004 The role of ergosterol and long-chain fatty acids of phytosphingolipids as membrane raft components in yeast, Saccharomyces cerevisiae. T Bunde, G Willhite, S Wright TAS 114 th Annual Meeting, Nov. 19, 2004 Localization of Ste2p, a G protein-coupled receptor, to lipid microdomains in Saccharomyces cerevisiae. M Murphy#, J Smith#, G. Willhite TAS 114 th Annual Meeting, Nov. 19, 2004 Ergosterol depletion leads to reduced Ste2p activity in Saccharomyces cerevisiae. N. Billings#, A. Dalton#, G. Willhite TAS Regional Meeting, Apr. 23, 2004 Guest Lectures Determining the role of the lipid environment in the function of a G protein-coupled receptor, Ste2p, in Saccharomyces cerevisiae. G. Willhite TWC Alpha Chi Lecture Spring 2004 Bioterrorism. History of Terrorism (H 461r) taught by D. Duncan. Oct. 22, 2003 . Grants Received Lee B. Ledford Scholarship for Students in Appalachia , Determination of lipid-protein interactions using a GPCR in yeast (Saccharomyces cerevisiae), March 2004 Sigma-Xi Grant in Aid of Research, Development of a phospholipase A 2 inhibition assay as a tool to determine the mechanism of adaptive immunity in Periplaneta americana (the American cockroach), January 2001 University Research Council Award, University of Cincinnati, Isolation of an antigen binding factor in the American cockroach by preparative electrophoresis and affinity chromatography, June 1999 Professional Organizations Appalachian College Association Science Group, 2002-present Tennessee Academy of Science, 2004-present Honors and Awards Lockmiller Outstanding Teacher Award, Tennessee Wesleyan College, 2003-2004 Faculty Incentive Award, Tennessee Wesleyan College, 2003-2004 Biology Outstanding Teaching Assistant Award, University of Cincinnati, 2000
	Tennessee Wesleyan College, PO Box 40, Athens, TN 37371-0040 1-800-PICK-TWC ~ (423) 745-7504